Plasmid
Part:BBa_K5407000:Design
Designed by: Fangyuan Duan Group: iGEM24_YiYe-Wuhan (2024-09-15)
pLKO.1 puro plasmid
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1645
Illegal SpeI site found at 1182 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1645
Illegal SpeI site found at 1182
Illegal NotI site found at 1931
Illegal NotI site found at 2829 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1645
Illegal BglII site found at 107
Illegal BglII site found at 148
Illegal BglII site found at 214
Illegal BglII site found at 3501
Illegal BamHI site found at 925
Illegal XhoI site found at 1444
Illegal XhoI site found at 1634
Illegal XhoI site found at 1938 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1645
Illegal SpeI site found at 1182 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1645
Illegal SpeI site found at 1182
Illegal NgoMIV site found at 6296
Illegal AgeI site found at 1669 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 426
Illegal BsaI.rc site found at 125
Illegal BsaI.rc site found at 1340
Illegal BsaI.rc site found at 3519
Illegal BsaI.rc site found at 5120
Illegal SapI site found at 2866
Illegal SapI site found at 4037
Design Notes
1
Source
1