Plasmid

Part:BBa_K5407000:Design

Designed by: Fangyuan Duan   Group: iGEM24_YiYe-Wuhan   (2024-09-15)


pLKO.1 puro plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1645
    Illegal SpeI site found at 1182
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1645
    Illegal SpeI site found at 1182
    Illegal NotI site found at 1931
    Illegal NotI site found at 2829
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1645
    Illegal BglII site found at 107
    Illegal BglII site found at 148
    Illegal BglII site found at 214
    Illegal BglII site found at 3501
    Illegal BamHI site found at 925
    Illegal XhoI site found at 1444
    Illegal XhoI site found at 1634
    Illegal XhoI site found at 1938
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1645
    Illegal SpeI site found at 1182
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1645
    Illegal SpeI site found at 1182
    Illegal NgoMIV site found at 6296
    Illegal AgeI site found at 1669
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 426
    Illegal BsaI.rc site found at 125
    Illegal BsaI.rc site found at 1340
    Illegal BsaI.rc site found at 3519
    Illegal BsaI.rc site found at 5120
    Illegal SapI site found at 2866
    Illegal SapI site found at 4037


Design Notes

1


Source

1

References